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PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.
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16

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

ENCOMPASS_PREFERRED
Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
Bacterial or Yeast Strain TOP10
Cell Type Chemically Competent
17

Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli

Provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into a plasmid vector. Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli is available in 20- and 40-reaction kit sizes.

ENCOMPASS_PREFERRED
Promoter T7
Content And Storage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
Product Type Cloning Kit
Cloning Method TA Cloning
Bacterial or Yeast Strain TOP10
For Use With (Application) PCR Cloning
Product Line One Shot
Cell Type Chemically Competent E. coli
18

Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

ENCOMPASS_PREFERRED
19

Invitrogen™ pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli

Use highly efficient, 5-minute cloning strategy to directionally clone a blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

ENCOMPASS_PREFERRED
20

Invitrogen™ Zero Blunt™ PCR Cloning Kit

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

ENCOMPASS_PREFERRED
Promoter T7
Content And Storage Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt PCR
Bacterial or Yeast Strain TOP10
Vector Blunt DNA Cloning Vectors
Product Line Zero Blunt
21

Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli

Quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

ENCOMPASS_PREFERRED
Promoter T7
Content And Storage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
Product Type Cloning Kit
Cloning Method TA Cloning
Bacterial or Yeast Strain INVαF ́
For Use With (Application) PCR Cloning
Product Line One Shot
Cell Type Chemically Competent E. coli
22

Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

ENCOMPASS_PREFERRED
Promoter T7
Content And Storage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
Product Type Cloning Kit
Cloning Method TA Cloning
Bacterial or Yeast Strain Not Included
For Use With (Application) PCR Cloning
Vector pCR2.1
Product Line TA Cloning
23

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli

Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing

ENCOMPASS_PREFERRED
24

Invitrogen™ GeneRacer™ Kit with SuperScript™ III RT and Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing

Advanced RACE (rapid amplification of cDNA ends) technique that improves efficiency of amplifying full-length 5' and 3' cDNA ends

ENCOMPASS_PREFERRED
25

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli

Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform

ENCOMPASS_PREFERRED
26

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing

ENCOMPASS_PREFERRED
27

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

ENCOMPASS_PREFERRED
Promoter T7, SP6
Content And Storage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Bacterial or Yeast Strain DH5α
Vector TOPO-TA Cloning Vectors
Product Line One Shot
28

Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

ENCOMPASS_PREFERRED
Promoter T7
Content And Storage Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt PCR
Bacterial or Yeast Strain Not Included
Vector Blunt DNA Cloning Vectors
Product Line TOPO, Zero Blunt
29

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Electrocomp™ E. coli

Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing

ENCOMPASS_PREFERRED
Promoter T7, T3
Content And Storage Box 1:
• Topoisomerase I-activated pCR™4-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Bacterial or Yeast Strain TOP10
For Use With (Application) Chromatin Biology
Vector TOPO-TA Cloning Vectors
Product Line One Shot
30

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli

Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing

ENCOMPASS_PREFERRED