PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.

16 – 30 of 134 results

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells

Pricing & Availability
Specifications

Antibiotic Resistance Bacterial	Ampicillin (AmpR), Kanamycin (KanR)
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Product Line	TOPO

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 Chemically Competent E. coli (Supply Center Only)

Designed for cloning PCR products directly from PCR reaction in 5 minutes

Pricing & Availability

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

Pricing & Availability

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Electrocomp™ E. coli

Each kit uses the pCR™4Blunt-TOPO; vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction

Pricing & Availability
Specifications

Promoter	T7, T3
Content And Storage	Box 1: • Topoisomerase I-activated pCR™4Blunt-TOPO™ vector • Salt solution • dNTPs • Control template • T3, T7, M13 forward, and M13 reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™
Bacterial or Yeast Strain	TOP10
For Use With (Application)	Chromatin Biology
Vector	Blunt DNA Cloning Vectors
Product Line	One Shot

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Pricing & Availability

Invitrogen™ Bac-to-Bac™ C-His TOPO™ Cloning Kit

Bac-to-Bac™ C-His TOPO™ Cloning Kit

Pricing & Availability

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1^R Chemically Competent E. coli (Supply Center Only)

Designed for cloning PCR products directly from PCR reaction in just 5 minutes

Pricing & Availability

Invitrogen™ pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1^R Chemically Competent E. coli

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector

Pricing & Availability

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1^R E. coli and PureLink™ Kit

Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform

Pricing & Availability

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1^R E. coli

Delivers fast, efficient and reliable cloning into various destination vectors for multitude of applications

Pricing & Availability

Invitrogen™ Bac-to-Bac™ HBM TOPO™ Cloning Kit

Bac-to-Bac™ HBM TOPO™ Cloning Kit

Pricing & Availability

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 Electrocomp™ E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

Pricing & Availability
Specifications

Content And Storage	Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	TOP10
Vector	TOPO-TA Cloning Vectors
Product Line	One Shot

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells

For fast, efficient cloning and subsequent in vitro transcription/translation

Pricing & Availability
Specifications

Promoter	T7, SP6
Content And Storage	Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid. Store at -68 to -85°C.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	TOP10
Cell Type	Chemically Competent

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F' Chemically Competent E. coli

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Pricing & Availability
Specifications

Promoter	T7, SP6
Content And Storage	Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid. Store at -68 to -85°C.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	TOP10F´
Vector	TOPO-TA Cloning Vectors
Product Line	One Shot
Cell Type	Chemically Competent

Invitrogen™ TOPO™ TA Cloning™ Kit, with One Shot™ MAX Efficiency™ DH5α-T1^R E. coli

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Pricing & Availability
Specifications

Promoter	T7, SP6
Content And Storage	Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	DH5α
Vector	TOPO-TA Cloning Vectors
Product Line	One Shot

PCR Cloning Kits

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