PCR Cloning Kits
Invitrogen™ TOPO™ TA, Dual TOP10 (Supply Center Packaging)
For fast, efficient cloning and subsequent in vitro transcription/translation
Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli
Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli
Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing
Invitrogen™ RNase T1 (1 U/μL)
Invitrogen™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites.
Invitrogen™ GeneRacer™ Kit with SuperScript™ III RT and TOPO TA Cloning™ Kit for Sequencing
Ensures amplification of only full-length transcripts via elimination of truncated messages from amplification process
Invitrogen™ GeneRacer™ Kit with AMV RT and TOPO TA Cloning™ Kit for Sequencing
Advanced RACE (rapid amplification of cDNA ends) technique that improves efficiency of amplifying full-length 5' and 3' cDNA ends
Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli and PureLink™ Kit
Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform
Invitrogen™ pENTR™/TEV/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli
Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector
Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli
Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System
Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli
Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli
Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Electrocomp™ E. coli
Offers the fastest and easiest method for high-efficiency cloning of blunt-end PCR products amplified with proofreading thermostable polymerases
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Electrocomp™ E. coli
Each kit uses the pCR™4Blunt-TOPO; vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction
Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning
Combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO vector to allow easy, high-efficiency cloning of blunt-end PCR products